Novel Synthesis Method of Kanamycin-Functionalized Gold Nanoparticles
- Start Date:- 2025-06-30
- End Date:- 2025-07-01
- Start Time:- 17:42:00
- End Time:- 17:42:00
Event Information :
With the rapid increase in cases of multidrug-resistant (MDR) bacteria worldwide, there is a huge demand for developing new generations of antibiotics to combat these bacteria. As an alternative to traditional drug discovery pathways, we designed an effective antimicrobial agent by modifying the existing commercial antibiotic kanamycin and conjugating it to the surface of gold nanoparticles (AuNPs).
Synthesis of Kanamycin-Gold Nanoparticles (Kan-AuNPs): Dissolve 1.72 mM kanamycin sulfate in M9 minimal culture medium buffer (pH 7.2 ± 0.2) and heat to 80 °C. Add aqueous KAuCl4 to achieve a final concentration of 0.79 mM KAuCl4. Incubate the mixture for 5 minutes, then cool it to room temperature. The color change from colorless to pink indicates the formation of Kan-AuNPs. Wash and centrifuge the mixture at 15,000 rpm for 20 minutes at least five times to remove unreacted kanamycin (Kan). After the final wash, the Kan-AuNPs are pelletized, freeze-dried, and stored at room temperature. The freeze-dried Kan-AuNPs are then re-suspended in autoclaved nanopure water for subsequent analysis.
Register at
Novel Synthesis Method of Kanamycin-Functionalized Gold Nanoparticles
- Start Date:- 2025-06-30
- End Date:- 2025-07-01
- Start Time:- 17:42:00
- End Time:- 17:42:00
Event Information :
With the rapid increase in cases of multidrug-resistant (MDR) bacteria worldwide, there is a huge demand for developing new generations of antibiotics to combat these bacteria. As an alternative to traditional drug discovery pathways, we designed an effective antimicrobial agent by modifying the existing commercial antibiotic kanamycin and conjugating it to the surface of gold nanoparticles (AuNPs).
Synthesis of Kanamycin-Gold Nanoparticles (Kan-AuNPs): Dissolve 1.72 mM kanamycin sulfate in M9 minimal culture medium buffer (pH 7.2 ± 0.2) and heat to 80 °C. Add aqueous KAuCl4 to achieve a final concentration of 0.79 mM KAuCl4. Incubate the mixture for 5 minutes, then cool it to room temperature. The color change from colorless to pink indicates the formation of Kan-AuNPs. Wash and centrifuge the mixture at 15,000 rpm for 20 minutes at least five times to remove unreacted kanamycin (Kan). After the final wash, the Kan-AuNPs are pelletized, freeze-dried, and stored at room temperature. The freeze-dried Kan-AuNPs are then re-suspended in autoclaved nanopure water for subsequent analysis.
Register at